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Only homozygous animals were being Utilized in the analyses, although heterozygous mice had been run in behavioral experiments. A few cohorts of eleven–sixteen mice underwent the first battery of behavioral assays for a total of 42 animals. All mice ended up initially housed with their littermates of precisely the same sexual intercourse during the Vanderbilt Murine Neurobehavioral Main, with managed twelve h mild/darkish cycles, freely offered food stuff and h2o, and managed temperature and humidity. The experimenter was in the beginning blinded into the genotype of every animal, and each animal underwent the same battery of behavioral checks to determine phenotype.
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